Fine Needle Aspiration Of Breast Masses


Article Author:
Jesse Casaubon


Article Editor:
John-Paul Regan


Editors In Chief:
Tod Aeby


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Orawan Chaigasame
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Abdul Waheed
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Radia Jamil
Patrick Le
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Hassam Zulfiqar
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Heba Mahdy
Ahmad Malik
Mark Pellegrini
James Hughes
Beata Beatty
Nazia Sadiq
Hajira Basit
Phillip Hynes
Tehmina Warsi


Updated:
3/13/2019 6:06:30 PM

Introduction

Fine needle aspiration (FNA) is a type of biopsy that is performed with a small (21 to 25 gauge) needle to obtain samples of tissue and fluid from solid or cystic breast lesions. It is one of the many different modalities for diagnosing breast masses outside of formal excision. Other methods include core needle biopsy (CNB) and Mammotome vacuum-assisted stereotactic biopsy. FNA and CNB can be performed with the assistance of imaging guidance (namely ultrasound) or done “free-hand” in the case of palpable breast lesions. Vacuum-assisted biopsy is performed with sonography, mammography, or MRI assistance.[1][2][3][4] Controversy exists regarding which is the preferred method for diagnosing, as each has strengths and weaknesses, and their incorporation into the breast surgeon’s practice varies. FNA has many advantages and potential uses making it a popular option for the diagnosis and treatment of certain breast lesions.

Anatomy

The breasts of an adult female are located on the anterior portion of the chest wall and play a vital role in milk production. The breast tissue lies on the pectoralis major muscle and is supported by many ligaments that arise from either side of the sternum. Each breast consists of 20 lobes that are dispersed evenly below the nipple. Surrounding each lobe is fatty tissue that gives the breast its shape and size. Each of the lobes consists of numerous lobules which produce milk in response to prolactin and the female sex hormones. The female breast can develop cystic and solid lesions in the lobes. In general, the majority of cystic lesions are benign, and the majority of solid lesions are malignant. Breast cysts tend to develop at the terminal end of the ducts and accumulate fluid.[5]Most breast cysts are small ranging in diameter from 5 mm to 20 mm and may fluctuate in size over the course of the menstrual cycle. A female may develop one or multiple cysts. Most of these cysts are painless, but some women may have discomfort or discover them during palpation of the breast.

Breast cysts are strongly influenced by hormones and are most common in women of childbearing age. Because of the concern of malignancy, women often seek medical attention. [6][7] While a breast cyst may be palpable, sometimes it can be confused with a solid mass. Hence, one of the first approaches to management is a fine needle aspiration biopsy. Some physicians will even order an ultrasound before the FNA to determine the depth, number, and location of the cyst or cysts.

Indications

Diagnosis

FNA is indicated for patients with a mammographic abnormality or palpable breast lesions. FNA remains controversial for the diagnosis of suspected malignant lesions due to the relatively small amount of breast tissue that is sampled and the high rate of non-diagnostic or inadequate samples. In this case, the “triple test” is performed using a combination of the physical exam, imaging studies and cytology to make this diagnosis to increase diagnostic accuracy. Though the Z0011 study is changing the treatment of axillary disease, axillary ultrasound with FNA sampling of suspicious appearing nodes is advisable.[8][9]

Therapeutic

FNA is indicated for breast cysts that are large, symptomatic, display vegetations, or imaging abnormalities.[10]

Contraindications

FNA should not be done in the following situations:

  • Presence of a skin infection
  • Patient is on anticoagulant therapy
  • Patient has a bleeding disorder
  • Patient does not give consent
  • Presence of a coagulopathy
  • The lesion is deep and not palpable

Equipment

The technique uses alcohol swabs, povidone-iodine solution, sterile gloves, and drapes, a 21 gauge needle with a semiopaque needle hub and a 3 ml or 5 ml syringe. A plastic bandage with soft gauze is needed to close the hole. The use of local anesthesia is not necessary unless the patient is anxious. However, if the lesion is deep and one is going to make multiple attempts, 1% lidocaine should be infiltrated in the skin area.

Preparation

Fine needle aspiration can be done in a clinic or operating room. The procedure should be done in a strict sterile manner. The area of the breast should be prepped and draped in a sterile fashion. The performer should use sterile equipment including gloves.

Technique

FNA is performed percutaneously using a small needle (usually a 21 to 25 gauge). The skin is prepared with alcohol, chlorhexidine, or betadine then numbed with a local anesthetic. The mass is localized by palpation or by ultrasound guidance. The mass in question is punctured while negative pressure is created and maintained in the syringe (increasing diagnostic yield). Multiple passes of the needle are made through solid lesions. The puncture is repeated with slightly different needle trajectories to sample different territories. If the lesion is cystic, the liquid is aspirated fully. Then the samples are sent for evaluation in pathology. The area around the puncture site is check for evidence of bleeding or hematoma.

Complications

Even though FNA appears to be a simple procedure, a number of complications can occur which include the following:

  • Bruising
  • Hematoma
  • Infection
  • Pneumothorax if the needle is advanced deep in the chest area
  • Pain

So far there is no evidence to show that FNA causes seeding of breast cancer or worsens survival or prognosis. More important, FNA does not affect the number of falsely positive mammograms as long as the radiologist has prior information of where the FNA was performed.

Clinical Significance

Advantages of FNA over other Modalities

  • Cost-effective (declines with inconclusive results/need for repeat biopsies)
  • Less invasive/less painful secondary to smaller needle size
  • Can be performed in the clinic or office
  • Appropriate for patients who are being anticoagulated with low risk of hematoma/bleeding
  • Can be used to sample axillary tissue

Disadvantages

Lower diagnostic yield than core needle biopsy

  • Four percent to 13% of samples found to be inadequate or nondiagnostic
  • More difficult to distinguish invasive versus in-situ carcinoma
  • May require more experienced cytopathologist for diagnosis
  • Non-diagnostic results necessitate repeat biopsy (typically CNB or vacuum-assisted)

Accuracy

Certain studies have cited the sensitivity of aspiration cytology for malignancy at 64% for one aspiration sample and 91% in patients with three samples. Specificity was 56% (inadequate or unsatisfactory cytological preparations.

Follow-up

If the FNA reveals a non-bloody aspirate and the cyst resolves, no more follow up is required. Some physicians do follow up with an ultrasound to ensure that the cyst has completely resolved. Re-evaluation is usually done 4 to 6 weeks later.

If the cyst persists after aspiration or contains bloody fluid, the patient should be referred to a surgeon for a formal biopsy.

Enhancing Healthcare Team Outcomes

FNA is a very common procedure done by the primary care provider, nurse practitioner, internist, radiologist, surgeon and many others. The key is to know the anatomy and potential complications. It is also important to know that FNA has a low yeild and thus a negative result should never be accepted if there is high suspicion of something more sinister. In such scenarios, it is important to communicate with other professionals on the best work up of the patient.[11]


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Fine Needle Aspiration Of Breast Masses - Questions

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The contents of a breast cyst have been aspirated under ultrasound guidance. It is bloody and purulent. What is the next step?



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Which is NOT an advantage of Fine Needle Aspiration (FNA) over Core Needle Biopsy (CNB) in the diagnosis of suspicious breast lesions?



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Which of the following is not a disadvantage of using fine needle aspiration biopsy over core needle biopsy of a breast mass?



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Which of the following is not a possible complication of fine needle aspiration (FNA) for a palpable breast mass suspicious for cancer?



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Which of the following is not an indication for cytologic evaluation after fine needle aspiration of a breast cyst?



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Fine Needle Aspiration Of Breast Masses - References

References

Yii N,Read T,Tan CC,Ng SL,Bennett I, Diagnosing phyllodes tumours of the breast: how successful are our current preoperative assessment modalities? ANZ journal of surgery. 2018 Oct;     [PubMed]
Brennan SB,D'Alessio D,Kaplan J,Edelweiss M,Heerdt AS,Morris EA, Positive predictive value of biopsy of palpable masses following mastectomy. The breast journal. 2018 Sep;     [PubMed]
Jairajpuri ZS,Jetley S,Rana S,Khetrapal S,Khan S,Hassan MJ, Diagnostic challenges of tubercular lesions of breast. Journal of laboratory physicians. 2018 Apr-Jun;     [PubMed]
Mišković J,Zorić A,Radić Mišković H,Šoljić V, Diagnostic Value of Fine Needle Aspiration Cytology for Breast Tumors Acta clinica Croatica. 2016 Dec;     [PubMed]
Ibikunle DE,Omotayo JA,Ariyibi OO, Fine needle aspiration cytology of breast lumps with histopathologic correlation in Owo, Ondo State, Nigeria: a five-year review. Ghana medical journal. 2017 Mar;     [PubMed]
Shirian S,Daneshbod Y,Haghpanah S,Khademi B,Noorbakhsh F,Ghaemi A,Mosayebi Z, Spectrum of pediatric tumors diagnosed by fine-needle aspiration cytology. Medicine. 2017 Feb;     [PubMed]
Giamanco NM,Jee YH,Wellstein A,Shriver CD,Summers TA,Baron J, Midkine and pleiotrophin concentrations in needle biopsies of breast and lung masses. Cancer biomarkers : section A of Disease markers. 2017 Sep 7;     [PubMed]
Ahmadinejad M,Hajimaghsoudi L,Pouryaghobi SM,Ahmadinejad I,Ahmadi K, Diagnostic Value of Fine-Needle Aspiration Biopsies and Pathologic Methods for Benign and Malignant Breast Masses and Axillary Node Assessment Asian Pacific journal of cancer prevention : APJCP. 2017 Feb 1;     [PubMed]
Ferré R,Omeroglu A,Mesurolle B, Sonographic Appearance of Lesions Diagnosed as Lobular Neoplasia at Sonographically Guided Biopsies. AJR. American journal of roentgenology. 2017 Mar;     [PubMed]
Delaloge S,Bonastre J,Borget I,Garbay JR,Fontenay R,Boinon D,Saghatchian M,Mathieu MC,Mazouni C,Rivera S,Uzan C,André F,Dromain C,Boyer B,Pistilli B,Azoulay S,Rimareix F,Bayou el-H,Sarfati B,Caron H,Ghouadni A,Leymarie N,Canale S,Mons M,Arfi-Rouche J,Arnedos M,Suciu V,Vielh P,Balleyguier C, The challenge of rapid diagnosis in oncology: Diagnostic accuracy and cost analysis of a large-scale one-stop breast clinic. European journal of cancer (Oxford, England : 1990). 2016 Oct;     [PubMed]
Kanchanabat B,Kanchanapitak P,Thanapongsathorn W,Manomaiphiboon A, Fine-needle aspiration cytology for diagnosis and management of palpable breast mass. The Australian and New Zealand journal of surgery. 2000 Nov;     [PubMed]

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