Physiology, Gastrin


Article Author:
Jordon Prosapio


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Ishwarlal Jialal


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Phillip Hynes


Updated:
11/26/2018 11:53:07 AM

Introduction

Gastrin is a peptide hormone primarily responsible for enhancing gastric mucosal growth, gastric motility, and secretion of hydrochloric acid (HCl) into the stomach. It is present in the G cells of the gastric antrum and duodenum. Gastrin is primarily released in response to vagal and gastrin-releasing peptide GRP) stimulation secondary to ingestion of peptides, amino acids, gastric distention, and an elevated stomach pH.[1][2] Conversely, gastrin release is decreased in response to paracrine inhibition by somatostatin and decreased stomach pH.

Gastrin is secreted into the blood and carried to the gastric fundus and cardia where the majority of HCl secreting parietal cells are found. HCl is necessary for the conversion of inactive pepsinogen to active pepsin, protein digestion in the stomach, as well as the release of cobalamin (vitamin B12) from its salivary R-protein carrier. The main clinical indication for assaying gastrin is to diagnose a gastrin-producing tumor, gastrinoma. Also, there appears to be data emerging that suggests gastrin might have a role in certain cancers such as gastric cancer.

Issues of Concern

G-cells secrete gastrin into systemic circulation, which allows delivery of gastrin to parietal cells and enterochromaffin-like (ECL) cells in the gastric fundus and cardia. Gastrin has also been found to interact with acinar cells of the pancreas, gastric smooth muscle cells, endocrine cells, and some white blood cells.

Gastrin stimulates the proliferation of gastric mucosal endocrine cells (parietal cells, ECL cells). This trophic quality of gastrin may be related to potentiation of some cancers.

Gastrin has been found to modulate immunity and inflammation via CCKB/gastrin receptor expressing white blood cells.  Specifically, CCKB expression has been recorded in some polymorphonuclear leukocytes (PMNs), mononuclear cells, and endothelial cells. It has demonstrated the ability to stimulate mast cell release of histamine, lymphocytic secretion of IL-2, endothelial expression of VCAM-1 and P-selectin, as well as endothelial secretion of IL-8.[3]

Cellular

G-cells are neuroendocrine cells responsible for the synthesis and secretion of gastrin. They are primarily found in the pyloric antrum but can also be found in the duodenum and the pancreas. They secrete gastrin when stimulated directly by vagal efferent neurons as well as GRP neurons. GRP neurons are stimulated by the presence of amino acids in the stomach, gastric distention, as well as vagal efferent stimulation. Gastrin secreted by these cells primarily acts on parietal cells and enterochromaffin-like cells (ECL cells) in the gastric pits of the stomach, but also interacts with gastric smooth muscle cells, acinar cells of the pancreas, lymphocytes, PMNs, and endothelial cells. Hence gastrin stimulates HCl secretion directly and by activating the ECL cells to secrete histamine which also results in HCL production.

Parietal cells, found in the pits of the gastric fundus and cardia, secrete hydrochloric acid into the lumen of the stomach via K/H ATPase on the apical (luminal) membrane. Gastrin binds cholecystokinin (CCK) B receptors on the basal membrane of parietal cells to induce expression of K/H ATPase. Increased expression and activity of the K/H ATPase results in increased secretion of hydrogen ions (gastric acid) into the stomach.

Enterochromaffin-like cells, found in the gastric pits of the fundus and cardia of the stomach, secrete histamine which then binds H2 receptors on the neighboring parietal cells and further potentiate the secretion of gastric acid by the parietal cells.

Acinar cells, found in the pancreas, are responsible for the synthesis, storage, and secretion of pancreatic digestive enzymes. Gastrin binds CCK2 receptors on the acinar cells, inducing secretion of the cell’s stored digestive enzymes.[4]

Development

The developmental biology of gastrin and somatostatin cells occurs in the antropyloric mucosa of the stomach.[5] Gastrin expressing cells (G-cells) develop from multi-hormonal pre-endocrine cells found in the regenerative isthmus of gastric pits. These pre-endocrine cells undergo asymmetric division, generating a gastrin expressing daughter cell and somatostatin-expressing daughter cell with each division. This asymmetric division promotes paracrine organization of these cells, enabling mature somatostatin-expressing D-cells to regulate the growth and activity of their neighboring gastrin-expressing G-cells. Maturation of D-cells and G-cells is regulated by multiple transcription factors containing homeobox motifs including Pdx-1, Pax 4, Pax 6, Isl-1, and Nkx6.1.

Gastrin is a linear peptide that is initially translated as preprogastrin. Preprogastrin undergoes post-translation cleavage which produces a family of gastrin peptides of variable lengths, the most common being 'Big' gastrin, which is a peptide comprised of 34 amino acids. The bioactivity of gastrin is preserved in a 5 amino acid sequence called “pentagastrin,” found at the 5 C-terminal of all members of the gastrin family, regardless of the overall size of the gastrin peptide. The 5 C-terminal amino acids of gastrin and cholecystokinin (CCK) are identical.[6]

Organ Systems Involved

Gastrin is primarily involved in the upper GI tract, specifically the stomach, and to a lesser degree the duodenum, and the pancreas. Gastrin primarily affects the ECL cells and parietal cells of the gastric fundus and cardia. Gastrin's role also affects the immune system via poorly understood mechanisms involving the CCKBR on monocytes, lymphocytes, and mast cells.

Mechanism

The effects of gastrin are primarily mediated via binding of the cholecystokinin (CCKB) receptor. The CCKB receptor is a G-protein coupled receptor which, upon activation by gastrin, starts downstream signaling via the phospholipase C - diacylglycerol + inositol trisphosphate - calcium - protein kinase C cascade.[7]

The CCKB receptor is primarily found in the central nervous system (CNS), and gastrointestinal (GI) system but is also expressed on endothelial cells and some white blood cells (WBC). 

CCK receptors can also induce signaling pathways through tyrosine kinase receptors to a more limited degree and have been demonstrated in the transactivation of EGFR.

Related Testing

The major indication for assaying gastrin is to diagnose Zollinger-Ellison syndrome which is a gastrin-producing tumor resulting in recurrent, treatment-refractory peptic ulcer disease and diarrhea.

However, pentagastrin does have other indications.

Parenteral injection with synthetic pentagastrin has been used as a diagnostic aid for carcinoid syndrome by inducing symptoms in patients who are difficult to discern due to minimal presence of symptoms. It has also been used in nuclear medicine to detect the presence of Meckel's diverticulum.

Pentagastrin-stimulated calcitonin test can be used in patients with normal calcitonin levels who are suspected to have medullary thyroid carcinoma. If positive, the patient’s serum calcitonin levels will elevate to levels significantly above the normal range. Also, pentagastrin-stimulated calcitonin release can be used postoperatively in these patients to detect any remaining calcitonin-secreting parafollicular cells (C-cells).[6]

Pathophysiology

Any pathological process leading to uninhibited secretion of gastric acid will lead to peptic ulcers disease. Several of these pathologic processes are due to uninhibited gastrin-mediated secretion of gastric acid.

Helicobacter pylori gastritis is associated with increased gastrin levels, likely secondary to the reduction in somatostatin secreting D-cells and subsequent dysregulation of gastrin secretion by G-cells.[8] This imbalance leads to decreased pH which consequently overwhelms gastric mucosal defenses, often resulting in gastric mucosal damage and formation peptic ulcers.

Hypochlorhydria secondary to destruction of gastric parietal cells in pernicious anemia is associated with increased activity of G-cells resulting in hypergastrinemia. Diffuse hyperplastic nodules found in the gastric mucosa of patients with pernicious anemia may be attributed to the trophic effect of gastrin on the gastric endocrine cells.[8]

Cessation of proton pump inhibitor (PPI) therapy by patients who have been on chronic PPI therapy often results in refractory gastritis. This is due to a paradoxical increase in gastrin in response to H/K ATPase inhibition by PPIs. Upon cessation of PPI therapy, uninhibited H/K ATPase become hyperactive in the presence of elevated gastrin levels resulting in gastritis. Fortunately, this refractory gastritis is self-resolving as gastrin levels will return to normal with continued cessation of PPI therapy.[9]

Due to the trophic effect of gastrin, elevated gastrin levels in patients on chronic PPI therapy also raises concern for potentiation of gastric cancer, as elevated gastrin levels have been associated with gastric carcinoid tumors arising from the gastric ECL cells.

Chronically elevated levels of gastrin also draw concern due to the trophic effect of gastrin and its association with gastrointestinal cancers. CCKB receptors are upregulated in gastric cancer, and in vitro studies have demonstrated the ability of gastrin to enhance proliferation of human gastric cancer cell lines as well as colonic cancer cell lines.[10][11] Furthermore, gastrin has shown angiogenic and anti-apoptotic characteristics in the setting of several malignancies including gastric cancer. Elevated gastrin levels also been associated with development of gastric carcinoid tumors arising from the gastric ECL cells.[12] In these CCKB receptor positive gastric cancers and gastric carcinoid tumors, therapeutic drugs such as lorglumide and devazepide, which target CCKB receptors, may be substantially beneficial.[13][14][15] Also, gastric carcinoid tumors are associated with decreased serum levels of vitamin B12. Therefore, serum vitamin B12 levels should be monitored in all patients on chronic PPI therapy. However much further work is needed to define strategies that target gastrin-CCKR as a therapeutic target.

Zollinger-Ellison Syndrome (ZES) is characterized by the presence of a gastrinoma, hypersecretion of gastric acid, peptic ulcers, and secretory diarrhea. A gastrinoma is an unregulated gastrin-secreting neuroendocrine tumor (NET). Biopsy of the tumor will reveal typical NET histological patterns that stain positive for chromogranin A, synaptophysin, and gastrin.

Unregulated secretion of gastrin by gastrinomas lead to hyperstimulation and hyperplasia of gastric mucosal parietal cells and ECL cells. Hyperplasia and hyperactivity of these cells result in excessive secretion of gastric acid into the stomach. The gastric acid subsequently overwhelms the defense mechanisms of the lining of the gastric mucosa causing ulceration of stomach and duodenum.

Diagnosis is confirmed by the presence of elevated fasting serum gastrin concentration far more than 100 pg/ml in association with increased basal gastric acid secretion and/or low gastric pH below 2.0. Another useful test to confirm the diagnosis is the secretin stimulation test which can result in an increase in gastrin to over 120 pg/ml over basal.[16] A normal fasting serum gastrin level virtually excludes ZES. Seventy percent to 75% of gastrinomas are sporadic; however, 25% to 30% of these tumors are associated with type 1 multiple endocrine neoplasia (MEN1).[17] Multiple imaging modalities are available to delineate the tumor including CT, MRI, somatostatin-receptor scintigraphy. Patients diagnosed for gastrinoma must be evaluated for MEN1 regarding family history, history of hypercalcemia, nephrolithiasis, or pituitary tumors, and labs must be performed to measure serum calcium, parathyroid hormone, and prolactin.

It is essential to manage the symptoms associated with elevated gastrin levels especially the peptic ulceration. Surgery is the only curative therapy for gastrinomas. It is recommended in patients with non-metastatic sporadic gastrinomas or MEN1-associated gastrinomas greater than 2 cm in diameter. Specifically, excision/enucleation of pancreatic gastrinomas is effective, while duodenal gastrinomas often require duodenectomy as there are usually multiple duodenal tumors present. Surgery is not recommended in MEN1 associated gastrinomas less than 2cm in diameter as surgery is rarely curative in the presence of MEN1.[18][19]

Non-surgical therapy for ZES is based on the treatment of symptoms and prevention of complications secondary to peptic ulcers. Proton pump inhibitors (PPI) are first-line medical therapy, as they directly decrease the secretion of gastric acid into the stomach, thus reducing the ability of the gastric acid to overwhelm the mucosal defenses and preventing the occurrence of peptic ulcers. An initial prospective study of patients with gastrinoma refractive to H2 blocker therapy, symptoms were resolved in 23 of the 29 total patients. Due to the high success in the management of these patients with PPIs, surgery is no longer required to manage hypersecretion of gastric acid.[20]

Clinical Significance

Management and therapy for the gastrin-related disease, as with most areas of medicine, requires a team-based, interprofessional approach. Hypergastrinemia secondary to Helicobacter pylori infections may be managed via treatment of the infectious disease, not requiring and endocrine specialist or surgical intervention. Conversely, a patient with a gastrinoma (ZES) must be managed by a coordinated team of endocrinologists, gastroenterologists, radiologists, and surgeons. Determining the cause of hypergastrinemia is also a team approach, requiring primary care, gastroenterologists, endocrinologists, pathologists, among others.


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Physiology, Gastrin - Questions

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Select the diagnosis that is not associated with elevated basal serum gastrin level.



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Which of the following hormones increases the release of gastric hydrochloric acid?



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A 47-year-old male with past medical history significant for myocardial infarction, congestive heart failure, and gastrointestinal reflux disease presents to the clinic because he is worried about something he read in a magazine about proton pump inhibitors causing cancer. He has been on daily omeprazole for 15 years and says it has been at least a decade since he last had an episode of heartburn. He plans to stop taking his omeprazole, and asks if stopping cold turkey will exacerbate his heart failure. What is the best response?



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Which of the following is consistent with multiple endocrine neoplasia type I (MEN1) in a patient with a gastrinoma?



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Which hormone stimulates gastrin secretion in patients with a gastrinoma?



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which of the following is incorrect concerning the Physiology of gastrin



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A female patient presents to the clinic complaining of a cough that is worse in the morning and at night. The cough has persisted for 6 weeks, is exacerbated when the patient is laying down and improves when the patient is sitting up or standing. The patient’s medications include omeprazole and sitagliptin. The patient admits that she ran out of omeprazole 3 months ago but says she has been too busy to renew her prescription. She is 5’2” and 153 lbs. The patient reports having tried several diets to reduce her heartburn and control her diabetes, but she admits to having very little success to this point. Physical exam reveals that the patient is afebrile, lungs are clear to auscultation, there is no cervical adenopathy, and there is no postnasal drip. The clinician determines that the cough is most likely secondary to uncontrolled gastric acid reflux. Which of the following describes the major role of gastrin in this disease?



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Physiology, Gastrin - References

References

Xiaoli L,Wu CW,Kim HY,Tian W,Chiang FY,Liu R,Anuwong A,Randolph GW,Dionigi G,Lavazza M, Gastric acid secretion and gastrin release during continuous vagal neuromonitoring in thyroid surgery. Langenbeck's archives of surgery. 2017 Mar     [PubMed]
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Hughes JW,Muegge BD,Tobin GS,Litvin M,Sun L,Saenz JB,Gyawali CP,McGill JB, HIGH-RISK GASTRIC PATHOLOGY AND PREVALENT AUTOIMMUNE DISEASES IN PATIENTS WITH PERNICIOUS ANEMIA. Endocrine practice : official journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists. 2017 Nov     [PubMed]
Martín-Alcolea M,Rodríguez-Hernández I,Aldea M,Rosas I,Juncà J,Granada ML, Chronic proton pump inhibition therapy in the diagnostic accuracy of serum pepsinogen I and gastrin concentrations to identify pernicious anaemia. Clinical biochemistry. 2017 Jun     [PubMed]
van Leeuwaarde RS,de Laat JM,Pieterman CRC,Dreijerink K,Vriens MR,Valk GD, The future: medical advances in MEN1 therapeutic approaches and management strategies. Endocrine-related cancer. 2017 Oct     [PubMed]
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Petersen OH,Ueda N, Pancreatic acinar cells: effect of acetylcholine, pancreozymin, gastrin and secretin on membrane potential and resistance in vivo and in vitro. The Journal of physiology. 1975 May     [PubMed]
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